国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-2922EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

簡(jiǎn)要描述:EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2922
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-15
  • 訪  問(wèn)  量:2068

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

ATCC® Number:CRL-2922™    Price:
Designations:EA.hy926

Depositors:CS Edgell

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞


Source:Tissue: somatic cell hybrid

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may gbe required for the transfer of this ATCC material. Anyone purchasin ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

Restrictions:For-profit institutions must obtain license from University of North Carolina, Chapel Hill.

Antigen Expression:Factor VIII-related antigen; Homo sapiens, expressed

DNA Profile (STR):Amelogenin: X

CSF1PO: 10,11,12

D13S317: 11

D16S539: 11,12

D5S818: 11

D7S820: 8,9,10

THO1: 6,8,9.3

TPOX: 8,9

vWA: 14,17



Comments:The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. [PubMed: 6407019] [PubMed: 2079463]

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.
Atmosphere: air, 95; carbon dioxide (CO2), 5
Temperature: 37.0°C


Subculturing:Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25 (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Transfer cell suspension to a centrifuge tube and spin at approximay 125 X g for 5 to 10 minutes. Discard supernatant.

  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(3) to 3 X 10(3) viable cells/sq. cm is recommended.

  7. Incubate cultures at 37C. Subculture when cell concentration reaches between 8 X 10(4) and 1 X 10(5) cells/sq. cm.
    Interval: Twice a week
    Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended
    Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete Growth medium, 95; DMSO, 5
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

0.25 (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++):ATCC30-2101

phosphate-buffered saline:ATCC 30-2200

Cell culture tested DMSO:ATCC 4-X

Erythrosin B vital stain solution:ATCC 30-2404

Trypan Blue vital stain solution:ATCC 30-2402



References:92902: Edgell CJ, et al. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80: 3734-3737, 1983. PubMed: 6407019

92903: Bauer J, et al. In vitro model of angiogenesis using a human endothelium-derived permanent cell line: contributions of induced gene expression, G-proteins, and integrins. J. Cell. Physiol. 153: 437-449, 1992. PubMed: 1280276

92904: Edgell CJ, et al. Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926. In Vitro Cell. Dev. Biol. 26: 1167-1172, 1990. PubMed: 2079463

92905: Rieber AJ, et al. Extent of differentiated gene expression in the human endothelium-derived EA.hy926 cell line. Thromb. Haemostasis 69: 476-480, 1993. PubMed: 8322270

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞


















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久国产精久久精产国| 中国熟妇色XXXX欧美老妇多毛| 欧美精品九九99久久在免费线 | 最近国语视频在线观看免费播放| 国产理论片午午伦夜理片2021 | 无码人妻精品一区二区三区下载| 成人免费毛片aaaaaa片| 精品人妻一区二区三区| 我和岳M愉情XXXX国产| 中国无码人妻丰满熟妇啪啪软件| 国产剧情av麻豆香蕉精品| 国内精品久久久久影院薰衣草| 国产V片在线播放免费无码 | 国产又粗又深又猛又爽又黄A片| 国产suv精品一区二区五| 免费无码又爽又刺激网站| 国产免费破外女真实出血视频 | 亚洲精品v天堂中文字幕| 97久久超碰国产精品2021| 国产欧美一区二区三区| 久久精品亚洲av无码四区毛片| 丰满雪白人妻人爽AV精品| 日产精品一卡2卡三卡4乱码| 色综合色综合久久综合频道88| 欧美VIVO18| 国语自产精品视频在线完整版| 久久精品人人做人人爽电影蜜月| 绯色AV视频精品一区二区| 少妇特黄A一区二区三区| 欧美重囗味成人无码区| 国产午夜视频在线观看 | 无码欧美人xxxxx日本漫画| 久久影院午夜理论片无码| 岛国激情一区二区三区| 亚洲av夜夜欢一区二区三区| 久久国产人妻一区二区| 午夜无码一区二区三区在线观看| 国产成人一区二区三区影院动漫| 国产精品视频一区二区三区不卡| 亚洲狠狠婷婷综合久久久久图片| 国偷自产AV一区二区三区123|