国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-2922EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

簡(jiǎn)要描述:EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2922
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-15
  • 訪  問(wèn)  量:2068

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

ATCC® Number:CRL-2922™    Price:
Designations:EA.hy926

Depositors:CS Edgell

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞


Source:Tissue: somatic cell hybrid

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may gbe required for the transfer of this ATCC material. Anyone purchasin ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

Restrictions:For-profit institutions must obtain license from University of North Carolina, Chapel Hill.

Antigen Expression:Factor VIII-related antigen; Homo sapiens, expressed

DNA Profile (STR):Amelogenin: X

CSF1PO: 10,11,12

D13S317: 11

D16S539: 11,12

D5S818: 11

D7S820: 8,9,10

THO1: 6,8,9.3

TPOX: 8,9

vWA: 14,17



Comments:The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. [PubMed: 6407019] [PubMed: 2079463]

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.
Atmosphere: air, 95; carbon dioxide (CO2), 5
Temperature: 37.0°C


Subculturing:Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25 (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Transfer cell suspension to a centrifuge tube and spin at approximay 125 X g for 5 to 10 minutes. Discard supernatant.

  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(3) to 3 X 10(3) viable cells/sq. cm is recommended.

  7. Incubate cultures at 37C. Subculture when cell concentration reaches between 8 X 10(4) and 1 X 10(5) cells/sq. cm.
    Interval: Twice a week
    Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended
    Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete Growth medium, 95; DMSO, 5
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

0.25 (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++):ATCC30-2101

phosphate-buffered saline:ATCC 30-2200

Cell culture tested DMSO:ATCC 4-X

Erythrosin B vital stain solution:ATCC 30-2404

Trypan Blue vital stain solution:ATCC 30-2402



References:92902: Edgell CJ, et al. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80: 3734-3737, 1983. PubMed: 6407019

92903: Bauer J, et al. In vitro model of angiogenesis using a human endothelium-derived permanent cell line: contributions of induced gene expression, G-proteins, and integrins. J. Cell. Physiol. 153: 437-449, 1992. PubMed: 1280276

92904: Edgell CJ, et al. Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926. In Vitro Cell. Dev. Biol. 26: 1167-1172, 1990. PubMed: 2079463

92905: Rieber AJ, et al. Extent of differentiated gene expression in the human endothelium-derived EA.hy926 cell line. Thromb. Haemostasis 69: 476-480, 1993. PubMed: 8322270

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞


















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美丰满熟妇xxxx性大屁股| 猫咪亚洲精品无码MV在线观看| 免费无码又爽又刺激a片| 欧美三日本三级少妇三级久久| 国模无码人体一区二区| 国产成人无码久久久精品一| 最近免费中文字幕大全免费版视频| 综合图区亚洲欧美另类图片| 国产亚洲精品资源在线26U| 欧美+国产+日产+韩国| 变态另类一区二区SM| 免费人成视频x8x8入口观看大| 亚洲一区二区三区无码国产| 免费看国产曰批40分钟| 国产色婷婷免费视频在线69堂| 亚洲熟悉妇女XXX妇女AV| 人妻无码av中文系列久久| 免费中国帅气体育生gary| 日本人妻丰满熟妇久久久久久| 亚洲A片无码精品毛片色戒 | 国产精品一区二区三区| 免费人做人爱视频| 国内精品久久久久久中文字幕| 久久99精品国产自在现线小黄鸭| 裸身美女无遮挡永久免费视频| 激情偷乱人伦小说免费看| 永久免费av无码网站性色av| 无永久免费精品精品永久-夜色 | 亚洲男人天堂| CAOPORN免费视频国产| 99精品国产在热久久婷婷| 777午夜精品久久av蜜桃小说 | 88国产精品欧美一区二区三区| 国产精品久久无码一区| 久久www免费人成人片| A级毛片无码免费久久真人软件| 久久久久国产精品熟女影院 | 国偷自产AV一区二区三区123| 亚洲av激情无码专区在线播放| 麻豆AV天堂一区二区香蕉| 国际精品鲁一鲁一区二区|