国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-9444MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

簡(jiǎn)要描述:MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫管理規(guī)范,提供 培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-9444
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:2145

產(chǎn)品分類

Product Category

詳細(xì)介紹

MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

ATCC® Number:CRL-9444™    Price:$329.00
Designations:MeT-5A

Depositors:The United States of America

Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Tissue: mesothelium
Cell Type: epithelialvirus transformed


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

Tumorigenic:No

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11,13

D16S539: 12

D5S818: 12

D7S820: 10

THO1: 6,9.3

TPOX: 8

vWA: 15,18



Comments:Mesothelial cells were isolated from pleural fluids obtained from non-cancerous individuals.

The cells were transfected with the pRSV-T plasmid (an SV40 ori- construct containing the SV40 early region and the Rous sarcoma virus long terminal repeat) and cloned.

The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc.

The cells stain positively for vimentin, keratins and SV40 T antigen.



Propagation:ATCC complete growth medium: Medium199 with Earle's BSS, .75 mM L-glutamine and 1.25 g/L sodium bicarbonate supplemented with with 3.3 nM epidermal growth factor (EGF), 400 nM hydrocortisone, 870 nM insulin, 20 mM HEPES, trace elements (see above reference) and 10% fetal bovine serum.
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:recommended serum:ATCC 30-2020

References:21937: Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989
23292: Lechner JF, et al. Asbestos-associated chromosomal changes in human mesothelial cells. Proc. Natl. Acad. Sci. USA 82: 3884-3888, 1985. PubMed: 2987952

























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品无码电影在线观看 | 午夜精品一区二区三区在线观看| 99久久99久久精品国产片果冻 | 亚洲av无码精品国产成人| 国产成人精品久久久久| 久久亚洲色WWW成人欧美| 激情亚洲一区国产精品| 久久久综合精品一区二区三区| 欧美性猛交久久久乱大交| 亚洲成色WWW久久网站| 午夜男女爽爽影院a片免费| 激情都市 校园 人妻 武侠 | 麻豆XXXX乱女少妇精品| 日本伊人精品一区二区三区| 欧美巨鞭大战丰满少妇| 国产午夜视频在线观看| 国产精品麻豆成人AV电影| 永久免费不卡在线观看黄网站| 久久爱综合久久爱久久爱| 日韩AV无码成人精品国产| 人人做天天爱夜夜爽 | 亚洲综合欧美色五月俺也去| 男男黄gay片免费网站www| 国产chinasex对白videos麻豆| 性a欧美片| 色一情一区二| 日韩av无码一区二区三区不卡| 这里只有精品在线视频| 成人毛片100免费观看| 一区二区亚洲精品国产精华液| 亚洲日韩国产av无码无码精品| 色精阁久艾草国产WWW| 99riav国产精品视频| 亚洲av一区二区无码波多野结衣| 国产一区二区精品丝袜| 性色av一区二区三区人妻| 欧美日韩在线播放二区| 美女黄网站视频免费视频软件| 国产成人无码精品久久久免费 | 亚洲精品乱码久久久久蜜桃| 国内精品久久久久精免费|