国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細胞庫>人正常細胞>HTB-103KATO III細胞, 人胃癌細胞

KATO III細胞, 人胃癌細胞

簡要描述:KATO III細胞, 人胃癌細胞
ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

  • 產(chǎn)品型號:HTB-103
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1932

產(chǎn)品分類

Product Category

詳細介紹

KATO III細胞, 人胃癌細胞

OrganismHomo sapiens, human
Tissue

stomach:derived from metastatic pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac

Product Formatfrozen
Morphologyspherical
Culture Propertiesmixed, adherent and suspension
Biosafety Level1
Diseasegastric carcinoma
Age55 years adult
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
KaryotypeThe stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected.
Clinical Data

55 years adult

Asian

male

TumorigenicYes
Effects

Yes, in cheek pouches of anti thymocyte serum treated hamsters

No, in nude mice

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
KATO III細胞, 人胃癌細胞
Subculturing

Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove culture medium with floating cells to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 2.. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 4. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximay 125 xg for 5 to10 minutes. 5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Place culture vessels in incubator at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: 5% CO2 in air recommended

Temperature: 37°C          






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美大成色WWW永久网站婷| 18GAY男同69亚洲帅男蓝宇| 日韩视频 中文字幕 视频一区| 国产精品无码一区二区三级| 国产AV无码专区亚洲AV| 免费看久久妇女高潮A| 亚洲无码在线播放| 久久AV色欲AV久久蜜桃麻豆| 久久精品水蜜桃av综合天堂 | 人妻 丝袜美腿 中文字幕| 精品欧洲av无码一区二区| 免费成人在线视频| 樱桃视频影视在线观看免费| 免费a级毛片在线播放| 人妻被按摩师玩弄到潮喷| 亚洲国产精品无码久久一区二区| 精品国产污污免费网站AⅤ| 亚洲av永久无码精品| 大香伊人久久精品一区二区| 国产chinasex对白videos麻豆 | 好男人资源在线看免费的| 欧美大荫蒂av高潮| 色偷偷88888欧美精品久久久| 亚洲JIZZJIZZ中国少妇| 凹凸久久人人澡超碰凹凸| 仙踪林果冻传媒一区二区| 亚洲午夜无码久久久久软件| 人妻 白嫩 蹂躏 惨叫| 24小时在线看免费观看直播 | 少女免费观看完整电视电影| 女人与拘做受全过程免费视频| 欧美激情视频| 日韩精品一区二区三区色欲AV| 国产超碰AV人人做人人爽| 无码人妻一区二区三区在线| 国产精品免费a v片在线观看| 成全视频免费高清观看在线动漫| 欧美大浪妇猛交饥渴大叫| 美女张开腿给男人桶爽久久| 亚洲 欧洲 日产 经典| 日韩午夜理论免费TV影院|