国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1676WM-266-4細(xì)胞, 人黑素瘤細(xì)胞

WM-266-4細(xì)胞, 人黑素瘤細(xì)胞

簡(jiǎn)要描述:WM-266-4細(xì)胞, 人黑素瘤細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1676
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1668

產(chǎn)品分類

Product Category

詳細(xì)介紹

WM-266-4細(xì)胞, 人黑素瘤細(xì)胞

ATCC® Number:CRL-1676™    Price:$318.00
Designations:WM-266-4Depositors:M HerlynBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth aProperties:adherentOrganism:Homo sapiens (humn)Morphology:epithelial Source:Organ: skin Disease: melanomaCellular Products:proteoglycan antigen characteristic of melanomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.WM-266-4細(xì)胞, 人黑素瘤細(xì)胞Tumorigenic:YesDNA Profile (STR):Amelogenin: X CSF1PO: 12 D13S317: 12,13 D16S539: 11,12 D5S818: 13 D7S820: 8 THO1: 7,9 TPOX: 8,11 vWA: 15,17Cytogenetic Analysis:The cell line shares many properties with the parent tumor line including same karyology. This is a hypertriploid human cell line. The modal chromosome number is 82, occurring at 32%, with polyploidy at 8.6%. (Cells with 80 chromosome counts also occurred at a high frequency.) There were two paired [der(1)t(1;?) (p32;?), and der(11)t(2;11) (q22.1;p13)], and three to four single markers consistently found in each cell. Several other markers were also found in some cells only, including a small metacentric chromosome of unknown origin. Neither DMs nor HSRs were detected. Among the normal chromosomes, N11 had only single copy, and N1, N6, N8, N9, and N16 had two copies each. As expected, other normal chromosomes had three to four copies per cell. Two X chromosomes were generally found in each cell.Age:58 yearsGender:femaleComments:The WM-266-4 cell line was derived from a metastatic site of a malignant melanoma. Another cell line derived from the primary tumor of the same patient also is available (ATCC CRL-1675).Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol:                                          Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020derived from same individual:ATCC CRL-1675

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
视频分类 国内精品| 欧美xxxx做受欧美| 性欧美XXXXX乱极品少妇| 久久九九精品99国产精品| 亚洲av永久无码精品无码麻豆| 久久人人爽人人爽人人片AV高清| 久久久国产精品免费A片分环卫 | 丝袜 亚洲 另类 欧美 变态| 日本japanese熟睡人妻| 漂亮人妻被修理工侵犯| 成人做爰a片免费播放| 国产精品国产免费无码专区蜜桃| 亚洲午夜精品A片久久WWW解说| 99久久国产热无码精品免费| 亚洲精品www久久久久久| 国产精品一区二区久久不卡| 亚洲A片无码一区二区蜜桃久久| 久久婷婷五月综合色国产香蕉| 亚洲国产精品无码久久98| 国产成人精品综合久久久| 久久久久久久波多野结衣高潮| 色偷偷久久一区二区三区| 狠狠色综合网久久久久久| 日本精品videosse×少妇| 色屁屁www影院免费观看入口| 国产精品秘入口18禁麻豆免会员 | 色橹橹欧美在线观看视频高清| 欧美videosfree性派对| 熟女少妇丰满一区二区| AV大片在线无码永久免费| 国产一区二区三区日韩精品| 欧美老妇牲交videos| 日日躁夜夜躁狠狠久久av| 欧美日韩亚洲国内综合网| 久久久久久九九99精品| 无码88AⅤ欧美熟妇人妻影院| 秋霞影院午夜伦A片欧美| 国产精品亚洲AV色欲在线观看| 亚洲αv久久久噜噜噜噜噜| 人妻少妇精品视频一区二区三区| 亚洲AV成人无码久久精品老人|