国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-2741HBE135-E6E7人支氣管上皮細(xì)胞

HBE135-E6E7人支氣管上皮細(xì)胞

簡(jiǎn)要描述:HBE135-E6E7人支氣管上皮細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2741
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪(fǎng)  問(wèn)  量:3198

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

HBE135-E6E7人支氣管上皮細(xì)胞

細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說(shuō)明書(shū):Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.


HBE135-E6E7人支氣管上皮細(xì)胞

細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說(shuō)明書(shū):Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.



細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說(shuō)明書(shū):Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.


Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells     under an inverted microscope until       cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping, do not agitate the cells     by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by pipetting gently.

To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

Discard supernatant and resuspend cells in fresh serum-free growth medium.  Add appropriate aliquots of cell suspension to new culture vessels.

Place culture vessels in incubators at 37°C.


Subc*tion Ratio: 1:3 to 1:4

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 3th edition, published by Alan R. Liss, N.Y., 1994.

培養(yǎng)條件:Keratinocyte-Serum Free medium with 5 ng/ml human recombinant EGF (do not filter) and 0.05 mg/ml bovine pituitary extract (Invitrogen, formerly GIBCO-BRL, Cat.  17005-042) and supplemented with 0.005 mg/ml insulin and 500 ng/ml hydrocortisone.
























產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久天天躁狠狠躁夜夜免费观看 | 精品少妇一区二区三区A片| 日日av夜夜添久久奶无码| 日本精品无码久久久久三级国产| 青草国产精品久久久久久| 国产精品一区二区久久| 国产真人无码作爱免费视频APP | 欧美超级乱婬视频播放| 亚洲国产AV无码一区二区三区| 在线播放免费人成毛片乱码| 熟妇人妻久久中文字幕 | 国产AV无码专区亚洲AV麻豆| 中文无码人妻有码人妻中文字幕| 国产亚洲精品久久久久婷婷图片| 国产真实办公室沙发午睡系列 | 乱公和我做爽死我视频免费| 好爽毛片一区二区三区色欲| 色偷偷色噜噜狠狠网站久久| 日本三级欧美三级人妇视频| 亚洲色欲色欲WWW在线成人网| 成人无码欧美大尺度视频| 麻豆人妻无码性色av专区| 99热这里只有精品| 少妇人妻一区二区三区视频| 久久亚洲精品11p| 亚洲日韩精品av成人波多野| 欧美国产日韩a在线视频| 国产精品永久免费视频| 性色av免费网站| 国产v亚洲v天堂无码久久久| 亚洲 欧美 另类 动漫 在线| 久久99久久99精品免观看吃奶| 亚洲AV片不卡无码久久| 欧美人与性动交α欧美精品| 1000部精品久久久久久久久| 5566视频一区二区三区| 久久久av男人的天堂| 女人夜夜春精品a片| 99精品福利国产在线导航| 国产精品欧美А∨无码播放| 三人性FREE欧美|