国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>NCI-H226人肺癌細(xì)胞株

人肺癌細(xì)胞株

簡(jiǎn)要描述:CRL-5826 NCI-H226 人肺癌細(xì)胞株 ,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):NCI-H226
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:2034

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-5826 NCI

-H226 人肺癌細(xì)胞株ATCC® Number: CRL-5826™

Designations: NCI-H226 [H226]

Depositors: AF Gazdar, JD Minna

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: Epithelial

CRL-5826 NCI-H226 人肺癌細(xì)胞株

Source: Organ: lung

Disease: squamous cell carcinoma; mesothelioma [23570]

Derived from metastatic site: pleural effusion

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. ephone (214) 699-8056, (214) 688-7233.

Isolation: Isolation date: March, 1980

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 13,14

D16S539: 9,12

D5S818: 11,12

D7S820: 8,10

THO1: 8,9.3

TPOX: 8,11

vWA: 17

Cytogenetic Analysis: modal number = 47; range = 32-88; del(p25)

Gender: male

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to3 days

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..


CRL-5826 NCI-H226


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
美女高潮无遮挡免费视频| 亚洲欧洲精品饰品推荐有限公司| 日日狠狠久久8888偷偷色| 日本巜侵犯人妻人伦| 国产成人午夜高潮毛片| 亚洲欧美精品aaaaaa片| 97亚洲狠狠色综合久久久久| 国产超碰人人做人人爱ⅴa| 久久国产加勒比精品无码| 日本精品一区二区三区试看| 农民人伦一区二区三区| 亚洲国产精品久久久天堂不卡海量| 国产又湿又紧又大又爽AV视频| 人妻[21p]大胆| 99热最新成人国产精品| 欧美又粗又硬又大免费视频Q| 新狼窝色AV性久久久久久| JIZZ性欧美2| 丰满人妻少妇久久久久久| 丰满人妻熟妇乱又伦精品软件| 国产亚洲精品无码AA在线观看| 国产精品一区二区久久不卡| 国产精品美女久久久久| 国产精品特级毛片一区二区三区| 成人免费又大又爽a片视频| 野花高清完整版免费观看视频大全 | 亚洲欧美在线观看| 国产人妻久久精品一区二区三区| 无人在线直播免费观看| 国产男男激情VIDEOSGAY| 我和岳M愉情XXXX国产| 最好免费高清视频在线观看| 日韩一区二区三区高清电影| 精品久久久久久中文字幕| 无码人妻久久一区二区三区| 寂寞人妻瑜伽被教练日| 日韩精品无码一区二区中文字幕 | 欧美ZC0O人与善交| 无码一区二区三区| 欧美人妻一区二区三区| 老熟妇高潮一区二区三区|