国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CCL-105SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

簡(jiǎn)要描述:CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CCL-105
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:1799

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞的介紹

CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

ATCC® Number: CCL-105™ Price: $323.00

Designations: SW-13

Depositors: A Leibovitz

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: adrenal gland

Tissue: cortex

Tumor Stage: grade IV

Disease: primary small cell carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation: Isolation date: August, 1971

Virus Susceptibility: Human poliovirus 1

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 9

D16S539: 12

D5S818: 12

D7S820: 8,10

THO1: 7,8

TPOX: 8

vWA: 17,19

Cytogenetic Analysis: modal number = 63; range = 45 to 65.

Approximay 10% of the cells examined possessed a pair of dicentric chromosomes.

Isoenzymes: G6PD, B

Age: 55 years

Gender: female

Ethnicity: Caucasian

Comments: Electron microscopic studies show many bulb gap junctions (BGJ).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

References: 22140: . . In Vitro 8: 443, 1973.

22526: Leibovitz A, et al. New human cancer cell culture lines. I. SW-13, small-cell carcinoma of the adrenal cortex. J. Natl. Cancer Inst. 51: 691-697, 1973. PubMed: 4765382

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23212: Lasfargues EY, Ozzello L. C*tion of human breast carcinomas. J. Natl. Cancer Inst. 21: 1131-1147, 1958. PubMed: 13611537

26278: Johnson RG, Sheridan JD. Junctions between cancer cells in culture: ultrastructure and permeability. Science 174: 717-719, 1971. PubMed: 4330805

26279: Pinto da Silva P, Gilula NB. Gap junctions in normal and transformed fibroblasts in culture. Exp. Cell Res. 71: 393-401, 1972. PubMed: 4339896

CCL-105


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产成人无码AV片在线观看| 欧美13一14娇小性| 老牛嫩草一区二区三区| 久久熟妇人妻午夜寂寞影院| a级毛片免费网站| 无码永久成人免费视频| 免费国产裸体美女视频全黄| 永久免费a∨片在线观看| 卡一卡2卡3卡精品网站| 国产AV无码专区亚洲AV毛网站| 欧美日韩人妻精品一区二区三区 | 国产精品一国产精品| 久久夜色精品国产噜噜麻豆| 亚洲欧美精品aaaaaa片| 久久无码av三级| 国产偷窥熟女精品视频大全| 欧美 丝袜 自拍 制服 另类| 国产人A片777777久久| 久久伊人色AV天堂九九小黄鸭| 国产成人久久精品激情| 久久精品国产亚洲av蜜臀色欲| 女人国产香蕉久久精品软件| 爽爽爽男女爽视频免费| 国产精品av在线| 欧美综合缴情五月丁香六月婷| 爱我久久免费观看高清| 强奷人妻日本中文字幕| 无码AV精品一区二区三区| 精品亚洲AV无码喷奶水LIER| 精品国产三级av一区二区三区| 欧美午夜一区二区福利视频| 国产精品久久久久9999| 精品少妇人妻AV免费久久洗澡| 免费看美女被靠到爽的视频| 日韩人妻无码av一二三区 | 久久久国产精品无码一区二区| 黄金网站免费下载安装| 人人爽久久爱夜夜躁一区| 久久久久免费看黄A片| 青青青伊人色综合久久| 亚洲AV无码乱码国产精品|