国产精品国产三级区别第一集,亚洲精品国产成人,精品人妻无码一区二区三区,人妻熟妇乱又伦精品视频

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-2279MS1 小鼠胰島內(nèi)皮細(xì)胞

MS1 小鼠胰島內(nèi)皮細(xì)胞

簡要描述:CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2279
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2230

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞 的詳細(xì)介紹

CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞

ATCC® Number:  CRL-2279™

Designations:  MS1 (MILE SVEN 1)

Depositors:   JL Arbiser

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped:  froz


Organism: Mus musculus (mouse)en

Medium & Serum:  See Propagation

Growth Properties: adherent

Morphology: endothelial


CRL-2279

Source: Organ: pancreas

Strain: C57BL/6

Tissue: islet of Langerhans; endothelium

Cell Type: SV40 transformed

Cellular Products: tissue inhibitor of bioreactive matrix metalloproteinase (high levels)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Receptors: vascular endothelial growth factor (VEGF), expressed

Comments: MS1 is a pancreatic islet endothelial cell line established in 1994.

Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) constructed and screened for resistance to G418.

Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL.

The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.

The cells are useful for the study of signal transduction of angigenic factors.

It is the only line known that can give rise to benign hemangiomas.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

51452: Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久精品国产亚洲AV高清| 国产精品www夜色视频| 伊人久久大香线蕉亚洲五月天| 亚洲精品久久久无码大桥未久 | 国产无遮挡裸体免费视频在线观看| 久久AV无码AV高潮AV不卡| 亚洲AV无码99久久久国精| 免费看污又色又爽又黄又脏小说| 国偷自产AV一区二区三区动漫| 少妇无码一区二区三区免费| 亚洲日韩精品欧美一区二区一| 高清欧美性猛交XXXX黑人猛交| 日韩人妻一区二区三区免费| 亚洲人成网亚洲欧洲无码久久| 国产人久久人人人人爽| 最近中文字幕在线看免费完整版| a片人人澡c片人人人妻| 欧美日本精品一区二区三区| 熟女人妻av粗壮巨龙| 国产色婷婷免费视频在线69堂| 美妙人妻系列100部| 欧美三级乱人伦电影| 免费人成视频在线观看| 精品一区二区三区免费视频| 国产一区二区三区在线观看| 国自产拍偷拍精品啪啪| 国产人妻精品一区二区三区不卡| 怡红院a∨人人爰人人爽| 国内精品伊人久久久久AV| 免费1级a做爰片观看| 国产大陆亚洲精品国产| 日韩AV在线观看| 又色又爽又高潮免费视频观看| 欧美日韩免费观看在线影片| 少妇高潮毛片免费看a片| 亚洲国产精品无码专区网站| 久久av高潮av无码av| 精品国产av 无码一区二区三区| 色欲老女人人妻综合网| 中文字幕av人妻一本二本| 人人妻人人澡人人爽欧美一区|